Journal: International Journal of Molecular Sciences

Article Title: New Approach to the Cryopreservation of GV Oocytes and Cumulus Cells through the Lens of Preserving the Intercellular Gap Junctions Based on the Bovine Model

doi: 10.3390/ijms25116074

Figure Lengend Snippet: Expression of Cx37 in oocytes and CCs after cryopreservation and in vitro maturation in different co-culture systems. ( A ) Relative mRNA expression of Cx37 in oocytes; ( B ) relative mRNA expression of Cx37 in CCs; ( C – S ) immunofluorescent staining of Cx37 (green) and nucleus counterstaining with DAPI (blue). ( C , D ) ImmCOCs; ( E ) negative control; ( F , G ) COCs; ( H , I ) cryoCOCs; ( R ) fCCs; ( S ) cryoCCs. Descriptions of the groups can be found in (Experimental Design). Different subscript letters on the graph mean statistically significant difference between the groups ( p ≤ 0.05).

Article Snippet: As primary antibodies, we used Cx37 antibody Cat. No. #42-4400 (ThermoFisher Scientific, Waltham, MA, USA) at a 1:100 ratio.

Techniques: Expressing, In Vitro, Co-Culture Assay, Staining, Negative Control

Journal: International Journal of Molecular Sciences

Article Title: New Approach to the Cryopreservation of GV Oocytes and Cumulus Cells through the Lens of Preserving the Intercellular Gap Junctions Based on the Bovine Model

doi: 10.3390/ijms25116074

Figure Lengend Snippet: Primers used for qRT-PCR of genes in bovine oocytes and cumulus cells.

Article Snippet: As primary antibodies, we used Cx37 antibody Cat. No. #42-4400 (ThermoFisher Scientific, Waltham, MA, USA) at a 1:100 ratio.

Techniques: TaqMan Assay